KMID : 0364820150510020108
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Korean Journal of Microbiology 2015 Volume.51 No. 2 p.108 ~ p.114
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Secretion of the iron containing superoxide dismutased of Streptomyces subrutilus P5
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Park Jae-Seung
Kim Jae-Heon
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Abstract
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We tried to analyze the growth time for secretion of the iron containing superoxide dismutase by comparing the intra- and extracellular enzyme activity from Streptomyces subrutilus P5 and analyze possible genetic information for this enzyme secretion. The mycelial dry weights and glucose concentrations in culture filtrates were determined during growth. Glucose was consumed rapidly during logarithmic growth phase and almost exhausted at 24 h of cultivation. While the intracllular activity of iron containing superoxide dismutase was first appeared at three hours, the extracellar activity of this enzyme appeared from 7.5 h of cultivation, early logarithmic growth phase. This early presence of the superoxide dismutase might not be the result of cell lysis but active secretion pathway. There was no information for signal peptide responsible for the enzyme secretion in sodF. However, we found a type three secretion box in the promoter region of sooFthat has been known for the genes of type ¥² secreted proteins in other bacteria. This is the first report on the possible existence of type ¥² secretion in Streptomyces.
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KEYWORD
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Streptomyces, extracellular secretion, superoxide dismutase, type III secretion
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